Singapore

Dental caries remains a persistent major oral healthcare burden worldwide with little significant improvement in dental healthcare in the last 30 years. Intraoral/biofilm-tooth pH is the single parameter that has demonstrated accurate assessment of the caries risk, reflecting the summative integrated outcome of the complicated interactions between the three etiological factors, namely microorganisms/biofilm, diet/carbohydrates, and tooth/saliva/host. However, there is no technology/system capable of probing both the micro-pH profile in dentin tissue and acidogenic oral biofilm simultaneously, with high spatial/temporal resolution of pathophysiologic acid attacks. Herein, a highly sensitive pH-responsive Hybrid NanoParticle (pH-NP) is developed coupled with an ex vivo tooth-biofilm caries model to simulate key cariogenic determinants/steps in a well-controlled platform to decipher the mechanistic complexity of caries initiation/formation. The pH-NP emits two fluorescence intensities that can change in opposite directions with pH to measure with a ratiometric output sensitivity of 13.4-fold a broad clinically-relevant pH range of 3.0–8.0. Using [H+], in addition to pH, to calculate the “area-under-curve” corroborates with the “minimum-pH” in semi-quantifying the demineralizing potential in each biofilm-dentin zones/depth. The data mechanistically elucidates a two-pronged cariogenic effect of a popular-acidic-sweet-drink, in inundating the biofilm/tooth-system with H+ from both the drink and the metabolic by-products of biofilm.

MATERIALS FOR HUMANITY (MH 21)

Imaging Spatiotemporal pH Changes in Biofilm-Dentin Microenvironments using pH-Responsive Hybrid Nanoparticles

technical paper

**Please view our Welcome to MH21 video.**

<center>
<figure class="video_container">
	 <iframe src="https://screencast-o-matic.com/watch/cr1UYHV1pcz?v=6&ff=1&title=0&controls=1" width=826 height=526 frameborder="0" allowfullscreen="true"> </iframe>
</figure>
	</center>


**Walkthrough Video**

Here is a Walkthrough showing how to navigate the conference and network with others 

<center>
<figure class="video_container">
	 <iframe src="https://screencast-o-matic.com/watch/cr1vnrV1paE?v=6&ff=1&title=0&controls=1" width=826 height=526 frameborder="0" allowfullscreen="true"> </iframe>
</figure>
	</center>




	 Organized by
 
<a href="https://mrs.org.sg/">
<img src="https://assets.underline.io/uploads/markdown_image/1/image/95d5789689d5f900b90e9fe54ea3cf0d.jpg"</a>

 
	
	 In association with
 
<a href="http://www.ntu.edu.sg/">
<img src="https://assets.underline.io/uploads/markdown_image/1/image/334cd171ad2fdee033c635b368e5e160.png"</a>
	<a href="https://www.nus.edu.sg/">
<img src="https://assets.underline.io/uploads/markdown_image/1/image/32e6f82c0440f7bb24c590f9f67821bd.png"</a>
		<a href="https://www.a-star.edu.sg/">
<img src="https://assets.underline.io/uploads/markdown_image/1/image/72557865b589cfa3d2a0243ffce4a270.png"</a>
	
 


	 Supported by
 
		 <a href="https://www.visitsingapore.com/mice/en/about-us/about-secb/">
<img src="https://assets.underline.io/uploads/markdown_image/1/image/6e679842ead62be522ba6a630b82d5c9.png"</a>

To access the MH 21 Conference, [please register](https://mh21.mrs.org.sg/event/da3f9651-429f-4763-81b2-ebd7311b6bc8/websitePage:206f7605-1731-41f9-8421-56d3890f398f)

MH 21 is arranged around 6 themes that focus attention on pressing needs that should be addressed to enhance the well-being of communities and provide a forum for presenting breakthrough discoveries.

Targeting Inflammatory Macrophages with Polysaccharide Nanocarriers

Thermogels are valuable soft materials for biomedical applications. At low temperatures, they remain as solutions which can be used to entrap therapeutic payloads such as drugs and proteins within their solvated polymer matrices. Upon injection into the body, higher body temperatures cause the solutions to gelate in situ at the site of injection, allowing localized placement for applications such as drug delivery. Herein, we present our three-component thermogelling polyurethane technology comprising of biocompatible polyethylene glycol (PEG), poly(propylene glycol) (PPG) and a third component, which can be poly(caprolactone) (PCL), poly(tetrahydrofuran) carbonate (PTHF) or poly[(R)-3-hydroxybutyrate-(R)-3-hydroxyhexanoate] (PHBHx), as a unique platform for biomedical applications. Our thermogels loaded with anticancer drugs were highly effective for inhibition of tumour growth in vivo using mice models. Docetaxel-loaded PEG-PPG-PHBHx thermogels showed enhanced anti-melanoma effects on solid melanoma tumours compared with the free drug, whilst exhibiting excellent biosafety and no apparent harm to organs. With doxorubicin-loaded PEG-PPG-PTHF thermogels, sustained drug release for 16 days in vivo successfully inhibited growth of hepatocellular carcinoma. Impressively, incorporating a dye capable of aggregation-induced emission into the polymer structure allowed it to be used to track in-vivo doxorubicin release status and gel degradation, revealing valuable insights on the drug transportation pathways. Recent efforts on PEG-PPG-PCL thermogels are concentrated on their unprecedented use in vitreoretinal surgery as a vitreous substitute and an internal tamponading agent. The thermogel showed long-term biocompatibility in rabbit vitrectomy models, and was an effective endotamponade in a non-human primate surgical retinal detachment model. This is likely attributed to the thermogel providing support for the retina and preventing subsequent re-detachments.

Thermogels for Biomedical Applications

Subcellular compartmentalization is a key feature of eukaryotic cells. Selectively targeting subcellular compartments, though holding many exciting opportunities for biomedicine, remains rather underdeveloped. Enzyme noncovalent synthesis (ENS), an approach that integrate enzymatic reactions and self-assembly, provides a new way for subcellular targeting. In this talk, we briefly introduce the development of supramolecular self-assemblies for targeting mitochondria, endoplasmic reticulum, Golgi apparatus, and cell membrane. We mainly focus on the use ENS, which spatiotemporally controls the formation of supramolecular assemblies, for subcellular targeting and its applications, such as developing novel cancer therapeutics.

Enzymatic Noncovalent Synthesis for Targeting Subcellular Organelles

Fluorescence imaging in the second near-infrared window (NIR-II, 1,000-1,700 nm) demonstrates unique advantages of high sensitivity, high temporal and spatial resolution, and excellent image quality in comparison to that in the first near-infrared window (NIR-I, 650-950 nm) due to low scattering of photons and zero background fluorescence in the NIR-II region. Currently, a variety of NIR-II fluorescence imaging probes, such as single-wall carbon nanotubes, quantum dots, rare-earth-doped nanoparticles, conjugated polymer nanoparticles, small molecules, and AIEgens, have been developed as significant imaging tools for basic and clinical research. In this report, we begin with briefly introducing the advances of NIR-II fluorescence molecular probes in the last 10 years, including the design, preparation, and NIR-II fluorescence molecular imaging applications of AIEgens and indocyanine green (ICG). Subsequently, we highlight the large-scale preparation of NIR-II imaging probes and their applications in large animals, including New Zealand white rabbits and cynomolgus monkeys. Finally, we prospect the opportunities and challenges of NIR-II fluorescent molecular imaging probes in basic and translational studies.

Advances in NIR-II Fluorescence Imaging Probes

It is well-known that luminescent materials have found applications in many fields, such as lighting, display, biomedical analysis and solar energy light conversion and so on. The NIR excitation light for upconversion nanoparticles have many advantages in biomedical application, such as good photostability, low or zero background luminescence, higher penetration depth and so on. NIR light can be used to excite upconversion nanoparticles to produce UV-vis light, which can be further employed to excite the prodrugs or photosensitizers to kill cancer cells. Various of therapy processes can be combined together in one system under NIR irradiation, thus increasing the treatment efficacy.



Rare Earth Up-conversion Luminescent Nanomaterials: Controllable Synthesis, Property Modification and Biomedical Application

Cancer, a major public health problem worldwide, is a death causing disease. Chemotherapy and radiotherapy are main treatments for cancer. However, their side effects and resistance limited their clinical use. On the other hand, nanomedicine, the area of interdisciplinary science, applies nanotechnology to health and medicine [1]. First, we demonstrate that how nanotechnology overcomes platinum drug resistance [2]. The platinum prodrug was designed and tethered onto surface of gold nanorods. The designed delivery system can overcome resistance through increasing drug uptake and decreasing drug deactivation. We also used nanomedicine strategy to deliver chemotherapy drugs, such as Histone deacetylase (HDAC) inhibitor, to sensitize radiotherapy through inhibiting DNA repair [3]. Second, we developed a multifunctional platform with controlled activation of platinum prodrug using near infrared (NIR) light and with real-time evaluation of drug efficacy [4]. The ultraviolet (UV) light sensitive platinum prodrug was synthesized and conjugated onto surface of upconversion nanoparticles (UCNPs). Upon absorbing NIR light, UCNPs convert it into UV light, which activates platinum prodrug, release active components and then induce cancer cells apoptosis. Meanwhile, an apoptosis-imaging probe was also conjugated onto UCNPs. Once NIR light irradiated UCNPs, cancer cells apoptosis was triggered, which unleashed the release of enzyme, caspase-3 and subsequently activate the imaging probe. Therefore, the direct imaging of apoptosis in living cells can be for real-time evaluation of drug efficacy. Finally, yet importantly, nowadays immunotherapy generates promising clinical data that have not seen before. It showed that combining radiotherapy and immunotherapy can enhance immune responses and can boost ‘abscopal effect’. Unfortunately, response rates for this strategy remain low. Herein we utilizes antigen-capturing nanoparticles (AC-NPs) to improve this strategy [5]. Our engineered AC-NPs can capture a set of protein antigens released post radiotherapy and it helped antigen presentation, which eventually induce enhanced immune responses and enhance ‘abscopal effect’.

Nanomedicine Strategies for Improving Chemoradiotherapy, Real-time Evaluating Drug Efficacy, and Enhancing Immunotherapy in Cancer Treatment

The unique optical properties of lanthanide-doped inorganic nanocrystals have rendered their potential usefulness as novel nanodrugs for many applications. Surface modification with organic dye molecules is a useful strategy to manipulate the optical properties of lanthanide-doped nanocrystals. The interaction between dye molecules and nanocrystals provides unprecedented possibilities to gain new optical phenomena from the organic-inorganic composite systems. The development of highly efficient lanthanide-based organic-inorganic nanoconjugates may overcome the existing limitations to the lanthanide-doped nanomaterials and stimulate applications in area such as light energy harvesting, light emission, biosensing, therapeutics and photocatalysis. Driven by this motivation, our group has recently devoted to the fabrication and mechanical studies of lanthanide nanocrystals with either unique crystal lattice structures or novel core-shell heterogeneous structures. We are trying to understand the energy transfer through the surface of lanthanide-doped nanocrystals for applying these materials in nanomaterial therapeutics.

Dye-coupled lanthanide nanocrystals for advanced optical biosensing and photodynamic therapy

Formidable progress has been recently achieved in the fabrication and characterization of disordered low dimensional materials with unprecedented properties. For instance, particular forms of disordered graphene (reduced graphene oxides) and other two-dimensional materials, obtained by chemical exfoliation techniques, have been found suitable to improve the performances of composite materials, with applications in energy, textiles, solar cells, thermoplastics etc. In parallel, catalytic vapor deposition methods have been used intensively to upscale the growth of two-dimensional materials at wafer size, with resulting material morphologies ranging from polycrystalline to fully amorphous phases. Recent reports on wafer-scale synthesis of ultrathin layered amorphous carbon and boron nitride, structurally dominated by sp2 hybridization but lacking crystalline orders, have shown superior performances of those materials as coating and interfacial components, providing a new platform for properties reinforcement in the industrial context.

Here, we present our computational strategies to achieve predictive modelling in this context. For the “computationally-assisted” material synthesis, we use machine learning to generate atomic interaction potential in an arbitrary disordered local environment and obtain realistic large-scale material configurations through classical molecular dynamics, while generic tight-binding models are generated based on resulting spatial atomic distribution and optimized DFT-inspired Hamiltonian parameters. Next, we employ efficient linear scaling quantum transport [1] (see also www.lsquant.org) to simulate structural models containing up to a billion atoms, hence demonstrating unprecedented supremacy to match predictive modelling with experimental scales, and therefore constituting decision-making tools for material design and industrial performances achievement. We illustrate our strategy on various examples including the (thermal) reduction process of graphene-oxides for recovering higher crystallinity, the correlation between the degree of disorder and resulting physical properties in polycrystalline materials [2], as well as amorphous phases of sp2 carbon members [3] and boron-nitride [4]. The obtained results provide scaling laws and guiding rules for researchers and engineers to target the proper material growth and integration conditions for reaching the upper limit of a given application objective, such as flexible conductors, spin channel, ultrathin heat spreaders, energy harvesters, insulating thermal barriers, or ultralow dielectric coefficient materials.
[1] Z. Fan et al. S. Roche, Physics Reports 903, 1-69 (2021)
[2] AW Cummings et al. Advanced Materials 26 (30), 5079-5094 (2014)
[3] A Antidormi, L Colombo, S Roche, 2D Materials 8 (1), 015028
[4] S. Hong, et al. A. Antidormi, S. Roche, M. Chhowalla, H.J. Shin & H.S. Shin, Nature 582, 511–514 (2020)

Multiscale Computational Methods for Exploring Electrical and Thermal properties of highly disordered Composites Materials: From Machine-learning based Molecular Dynamics to Linear Scaling Transport Methods

It has been well known that various kinds of lattice defects are ubiquitous in real materials and they govern mechanical properties of materials based on pioneering studies. While removal of detrimental influence by lattice defects has been one of the principal aim of recent studies [1-2], it is reported that further exploitations of those lattice defects for other functional properties is possible, where lattice defects play yet another role or more to control or even create resultant materials properties [3-6].
Aiming at modifying or even creating new properties that single crystals cannot acquire, we have carried out modeling of lattice defects for atomistic simulations including molecular dynamics and ab initio calculations [7-12]. Then, we identify the origin of the resultant thermal transport properties, thereby exploiting lattice defects for advanced materials [13-15] in the framework of “crystal defect cores” [16].
In this presentation, findings upon modeling of lattice defects, resultant transport properties and underlying mechanisms behind the properties will be discussed.

References:
[1] S.F. Chichibu, et al., Nat. Mater. 5 (2006) 810–816.
[2] Z. Chen, et al., Nat. Commun. 8 (2017) 14101.
[3] Y. Takashima, et al., Nat. Commun. 2 (2011) 168.
[4] A. Nakamura, KM, et al., Nat. Mater. 2 (2003) 453–456.
[5] Y. Oshima, AN, KM, Science. 360 (2018) 772–774.
[6] K. Matsunaga, TY, AN, et al., Acta Mater. 195 (2020) 645–653.
[7] T. Oyama, MY, KM, et al., Phys. Rev. B, 71 (2005) 224105.
[8] M. Yoshiya and T. Oyama, J. Mater. Sci., 46 [12] (2011) 4176-4190.
[9] B. Feng, et al., Nat. Commun., 7 (2016) 11079.
[10] T. Yokoi and M. Yoshiya, Physica B, Condensed Matter, 532 (2018) 2-8.
[11] T. Yokoi, AN, KM, Phys. Rev. Mater., 4 (2020) 014605.
[12] T. Yokoi, AN, KM, et al., Phys. Chem. Chem. Phys., 23 (2021) 10118.
[13] S. Fujii, MY, et al., Sci. Rep., 8 (2018) 11152.
[14] S. Fujii, TY, MY, Acta Mater., 171 (2019) 154-162.
[15] W. Sekimoto, SF, MY, Scr. Mater., 202 (2021) 113991.
[16] https://www.core.mp.pse.nagoya-u.ac.jp/en/

Modeling of Lattice Defects and Resultant Thermal Transport Properties of Materials

N-type transparent conductors (TCs) are key materials in the modern optoelectronics industry. Despite years of research, the development of a high-performance p-type TC has lagged far behind that of its n-type counterparts, delaying the advent of “transparent electronics” based on transparent p-n junctions.[1] Here, we computationally investigate three layered oxychalcogenide structural motifs to try to predict new p-type TCs, namely the [Cu2Ch2][A3B2O5] (325), [Cu2Ch2][A4B2O6] (426) and [Cu2Ch2][A2BO2] (212) structural motifs. Specifically, we have used a materials informatics approach (SMACT) to screen through the search space using low-cost heuristic tools.[2] This reduces the potential combinations from 1800 to a more computationally tractable 228, which then undergo DFT calculations to assess thermodynamic and dynamic stability. In this talk, I will present an update on how our search has predicted >50 novel semiconductors with potential applications ranging from TCs[3,] to photocatalysts,[4], solar absorbers and thermoelectrics.

[1] A. Walsh and J.-S. Park, The Holey Grail of Transparent Electroncs, Matter, 3, 604 (2020)
[2] D. W. Davies, K. T. Butler, A. J. Jackson, A. Morris, J. M. Frost, J. M. Skelton and A. Walsh, Computational Screening of All Stoichiometric Inorganic Materials, Chem, 1 617 (2016)
[3] B.A.D. Williamson, G.J. Limburn, G.W. Watson, G. Hyett, and D.O. Scanlon. Computationally Driven Discovery of Layered Quinary Oxychalcogenides: Potential p-Type Transparent Conductors?
[4] G. J. Limburn, M. J.P. Stephens, B. A. D. Williamson, A. Iborra-Torres, D. O. Scanlon and G. Hyett. Photocatalytic, structural and optical properties of mixed anion solid solutions Ba3Sc2−xInxO5Cu2S2 and Ba3In2O5Cu2S2−ySey, Journal of Materials Chemistry A, 8, 19887 (2020).


Premium content

Imaging Spatiotemporal pH Changes in Biofilm-Dentin Microenvironments using pH-Responsive Hybrid Nanoparticles

Downloads

Next from MATERIALS FOR HUMANITY (MH 21)

Targeting Inflammatory Macrophages with Polysaccharide Nanocarriers