Belgium

Autochthonous landraces or local varieties are plant genetic resources characterized by high genetic variability, specific adaptation to environmental growth condition, and the presence of health-promoting specialized metabolites. However, these varieties are at risk of extinction due to their replacement by genetically uniform modern varieties. Untargeted metabolomics, based on liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), is a powerful tool to explore metabolite diversity, promote the valorization, and support the conservation of these landraces. This study investigated the metabolite diversity of three autochthonous lentil landraces from Molise region (Italy) — Capracotta (CA), Rionero Sannitico (RS), and Agnone (A) — compared to one landrace from Umbria region (Italy) — Castelluccio di Norcia (CS) — and three bean landraces from Molise (Ciliegino, CI), and Basilicata (San Spanish Ciliegino, CI SP), respectively. Untargeted metabolomics allowed the detection of 662 and 344 differentially accumulated metabolic features (DAMfs) in lentil (Le) and bean (Be) landraces, respectively. Metabolite annotation, supported by molecular network analysis, assigned 53% and 54% of DAMfs to different metabolic categories, with flavonoids, amino acids, and cinnamic acids as the most represented. Multivariate statistical analyses (PCA, PLS-DA, and HCA) highlighted the distinctiveness of each landrace. The antioxidant, and antibacterial activities of legume seed extracts were evaluated, correlating bioactivity with their metabolic profiles. Ongoing enrichment analysis will further define landrace-specific metabolic features, enhancing the understanding of their health-promoting potential and supporting conservation efforts of traditional varieties.

SEB Conference 2025

In depth characterization of autochthonous bean (Phaseolus vulgarisL.) and lentil (Lens culinarisMedik) landraces from Apennine Italian regions

legume

landraces

metabolomics

You need to log in with the email address you registered with. Access credentials have been sent to your email. 

Please be sure to check your spam and other email folders if you do not see an email confirmation right away.

Please log in to explore this event.

If you see this page it means you are not logged in or registered.

If you feel like you see this message by mistake please make sure you are logged in with the email you registered with.

If you are not registered yet, you can do so [**here**](https://www.sebiology.org/events/seb-conference-prague-2024/registration2024.html). 

Please login or register

SEB Conference 2024

SEB’s Annual Conference is celebrated for creating the perfect composition of research, new discoveries and collaborative connections. Be creative and innovative by exchanging ideas and knowledge with masters of biology from all over the world.

technical paper

You need to log in with the email address you registered with. 

If you see this page it means you are not registered. You can register via [this link](https://www.sebiology.org/events/seb-annual-conference-antwerp-2025/registration.html). 

If you are registered and feel like you are seeing this message by mistake, please make sure you are logged in with the same email that you registered with. 

Please register!

Over-fishing is a consequential anthropogenic driver of demographic and phenotypic changes in aquatic fish populations. In Lake Malombe, Malawi, over the past 40 years, intense fishing pressure has led to local extinctions, dramatic population declines, and rapid life history shifts in several commercially important benthic cichlid species. In some of these species, size at maturation has decreased by approximately 50% within only 20 generations, suggesting a strong effect of either fisheries-induced evolution or phenotypic plasticity in response to human induced harvest pressure.
To disentangle the genetic and plastic components of this life history shift, we conducted a common garden experiment using offspring from populations experiencing contrasting fishing pressures. Juveniles from heavily fished and weakly fished populations were reared under standardised laboratory conditions to control for environmental effects and isolate genetic differences. We observed an accelerated growth, earlier maturation, but reduced reproductive investment in offspring from heavily fished populations, consistent with a rapid evolutionary responses to fisheries pressure. Importantly, because of the non-size selective fisheries in Lake Malombe these shifts in life history traits are driven by biotic interactions such as competition for mates and reproductive opportunities, with smaller, faster-maturing individuals potentially gaining an advantage in heavily fished populations.
This work and evolutionary genomic investigations we have carried out highlight how human-induced pressures reshape the genetic determination of life history strategies, with cascading consequences for intra- and interspecific interactions, population stability, and the long-term resilience of biodiversity hotspots like Lake Malawi.

Disentangling evolutionary and plastic responses to over-fishing of Malawi Cichlid fish in a common garden experiment

The pace-of-life hypothesis predicts that intrinsic traits such as rates of metabolism and growth influence behaviour, with high-metabolism individuals expected to be bolder, more aggressive, and more exploratory. These behavioural tendencies may increase predation risk by driving individuals to forage in riskier habitats. We tested this hypothesis in a freshwater fish community by integrating metabolic assays, growth analysis, stable isotope analysis, and acoustic telemetry. Using lakeside intermittent flow respirometry, we measured routine metabolic rate (RMR) as a proxy for baseline energy expenditure. Using biopsy, we back-calculated individual rates of growth and quantified stable isotope ratios (δ13 C and δ15 N) to infer diet composition and trophic position. Fish were then implanted with predation-sensing acoustic transmitters to track movement, habitat use, and predation events in a whole-lake acoustic positioning system (Ontario, Canada). The lake was divided into habitat zones (e.g., open water, vegetated shallows, nearshore areas) to assess how individuals used risky versus safe habitats. We studied prey species (bluegill Lepomis macrochirus, pumpkinseed Lepomis gibbosus) and a predator (largemouth bass Micropterus salmoides) to characterize metabolic-behavioural syndromes and their links to diet, growth, movement, and survival. This research rigorously tests the pace-of-life hypothesis and may inform conservation efforts by identifying traits that increase vulnerability to predation and environmental change.

Predicting the behaviour of wild fish using metabolism, growth, and diet

Plant hydraulics is a growing field that investigates links between the structure and the hydrodynamic function of plants, from cellular to ecosystem scales. Recently, a collaboration of plant hydraulic physiologists and palaeobotanists began re-examining the fossil record to identify the function of key elements of plant vascular structure at the time of their origin.Xylem conduits, which mediate water transport in vascular plants, are subject to blockage by vapour-phase embolism. Under drought, the uncontrolled spread of embolism through the xylem results in mortality through hydraulic failure. Vascular anatomy and morphology can facilitate or constrain embolism spread by affecting the connectivity of the xylem conduit network. Insofar as vascular structure affects the risk of hydraulic failure, it must be subject to selection by drought.Network analysis suggests that drought selection shaped the well-known trend of increasing vascular complexity with plant or organ size. Selection by hydraulic failure would thus have promoted the early diversification of primary vascular forms during the Silurian-Devonian Radiation of plant life onto land. The early Devonian emergence of secondary xylem (wood) conferred resistance to hydraulic failure on par with increasingly complex contemporaneous primary vascular anatomies. Woody growth was particularly effective at maintaining embolism resistance with increasing plant size, although the earliest known woods could not support hydraulically safe arborescent habit without further anatomical innovations. The ongoing reanalysis of the fossil record aims to reinterpret the function of emerging vascular structures with respect to hydraulic safety, ultimately setting the hydraulics of extant plants in a revised evolutionary perspective.

Selection by drought in the early evolution of plant vascular networks

A prominent feature of biodiverse habitats is the acoustic landscape. Yet very little is known about the acoustic landscapes of extinct environments, for example of a Jurassic Forest. One problem is that recovering the real acoustic features of extinct tetrapods is challenging, as their vocal organs do not readily fossilize. Conversely, the stridulating organs present in the sclerotised cuticle of some arthropods do fossilise well. For example, the stridulatory structures (a file, plectrum, and sound radiating membranes) in the forewings of male crickets and katydids are preserved in fossils, offering a unique opportunity to investigate species diversification and the acoustic landscapes of the past. Integrating experimental and numerical procedures, several katydid fossils (of nine species) were studied to recreate the acoustic landscape of the mid Jurassic (Inner Mongolia, China). Wing vibration and calling songs of these fossils were recovered and validated. Jurassic katydids used sharp pure-tones (musical) calls, which made localization by eavesdropping predators difficult. While 60% of these species communicate in the low audio range 5-7 kHz, one species exploited ultrasounds (>20kHz). Results imply that ultrasonic communication in insects was established long before the emergence of bats. The results extend current understanding of the Jurassic soundscape, revealing insect song and hearing diversity, but also highlighting the co-evolutionary pressures and constraints exerted by ultrasound sensitive eavesdropping insectivorous predators other than bats. Such arm race is proposed as one of the main pressures that boosted the evolution of the mammalian cochlea and the sophisticated ‘insect cochlea’ shown by living katydids.

The reconstruction of an extinct Jurassic soundscape

Jumping represent one of the most widely used modes of locomotion in the animal kingdom. However, this does not mean that the characteristics of jumps are universal; there are many different ways in which jumps are powered (actuated). In orthopterans there are two main methods of actuation; direct muscle actuation and latch mediated spring actuation. It has previously been suggested that spring actuated systems provide propulsive power to an animal, which in turn generates incredibly fast jumps. Clown hoppers (Eumastacidae) are a group of south American grasshopper which use their spring actuated laterally projected legs to achieve jump speeds of up to 3.1 m/s. Similar to the muscle actuated group of arboreal orthopterans (Phlugidini), the clown hopper will perform targeted jumps, although the level of control which they employ is less fine tuned. When clown hoppers are presented with 3 different overhead target heights (50 mm, 75 mm and 100 mm), there was a significant difference between the linear velocity (m/s) at take-off with jump speed increasing as target height increases. However, whilst there was a generalised decrease in angular velocity (rad/s), this was not significant suggesting that whilst the hoppers can control the extent to which the spring in their legs powers the jumps (represented by the proportionate change in speed of the jumps), the ability to control jump rotation at different heights remains obscure.

Introducing the Monkey Grasshopper, a spring-powered jumper with the ability to control its linear velocity

Human activities release increasing amounts of chemical contaminants into aquatic environments, raising concerns about their impact on wildlife. Many pollutants act as endocrine disruptors, interfering with hormone-regulated processes essential for growth, reproduction, and development. In fish, such disruptions affect skeletal formation, muscle function, and neural signaling, all of which also affect behaviour. While chemical exposure is indeed known to alter fish behaviour, such as reducing responses to light-dark stimuli, it remains unclear whether these effects stem from skeletal changes or physiological disruptions. To address this, we study how altered skeletal mineralization affects zebrafish swimming behaviour. Using larval zebrafish (Danio rerio), a well-described animal model for skeletal development, we first manipulated calcium availability during early development to induce either increased or decreased bone mineralization. Larvae raised in low-calcium conditions lacked bone mineralization and moved less in 24-hour observations. In light-dark transition assays, they also failed to respond properly. Since calcium is critical for bone formation, muscle contraction, and neural signaling, we extended our study to F0 knockout larvae. F0 knock-outs of enpp1 (promotes mineralization by generating inorganic pyrophosphate) and entpd5a (inhibits mineralization by hydrolyzing nucleotide triphosphates) were generated and validated using genotyping, skeletal staining, and elemental analysis. Swimming behaviour was quantified at 5 days post-fertilization using automated tracking. Even at this early stage, altered mineralization resulted in reduced locomotion. We anticipate that a better understanding of the effect of skeletal mineralization on behaviour will allow for a more thorough understanding of the effect chemical pollutants have on fish behaviour.

Behaviour to the bone: linking skeletal mineralization to behaviour in larval zebrafish (Danio rerio)

Historically relatively sheltered from human disturbance, Arctic regions now face increasing threats from expanding maritime routes and industrial activities. These pressures add to existing environmental stressors, raising concerns about the conservation of fragile polar ecosystems, which are particularly vulnerable to cumulative impacts such as ocean acidification (OA) and widespread pollution. Combined stressors may cause unpredictable non-additive effects on marine ectotherms. Furthermore, by using traditional approaches limited to whole-organism levels, such as metabolic rates and mortality, we have overlooked sublethal, early-signals of physiological failure occurring at the cellular level. Therefore, we aimed at exploring the molecular responses of a key Arctic zooplankton species to different environmentally-realistic doses of a toxic polycyclic aromatic hydrocarbon under an OA scenario. For this purpose, the metabolome and lipidome of field-collected copepodid IV specimens of the copepod Calanus glacialis were profiled after two and ten days of exposure within an orthogonal design of four pyrene concentrations (0, 10, 100 and 200 nM) and two levels of OA (ambient and low pH). Multivariate analyses evidence an interaction between OA and pyrene contamination over time, albeit pyrene is a major driver. OA seemingly shifts the metabolomic dose response to pyrene compared to ambient pH. Further integrative analyses will help to decipher the functional consequences of such molecular alterations, to identify early-signals of physiological failure. Implementing multilayer knowledge from such multifactorial experimental approaches will be critical in improving our ability to predict the fate of Arctic species in future, more disturbed polar oceans.

In the Search of Sublethal Biomarkers: Metabolomic and Lipidomic Profiling of Arctic Copepods Facing Ocean Acidification and Pyrene Pollution

Arbuscular mycorrhizal fungi (AM fungi) may facilitate plants during increasingly prevalent extreme stress events such as heatwaves and drought. Heatwaves are commonly characterised by the presence of extreme temperatures and frequently co-occur with droughts or water deficits. The combination of these stressors is deleterious for plants and the role of AM fungi in ameliorating this compound stress event is not fully understood, particularly within threatened communities such as grasslands. This study has investigated the role of field-collected AM fungi in facilitating plant responses to heat and water stress using a simulated heatwave event in a factorial greenhouse experiment to test how native grassland species respond. We found that the impact of heat stress combined with water stress on plant performance was shown to be greater than the additive effects of heat and water stress in isolation. AM fungi was shown to have variable influences on plant growth, fitness, and physiological responses but these influences were commonly at odds with previous publications on AM fungi with agricultural species. Most notably, AM fungi increased the germination success of seeds of one species grown following exposure to climate stress. The results of this study highlight the dynamic role of AM fungi and help us understand grassland responses to heatwave events in the face of increasing extreme climate events. Furthermore, this study emphasises the importance of building ecologically relevant ex-situ experiments to study and understand current global pressures facing natural ecological communities.

Interactions of Arbuscular Mycorrhizal Fungi with Native Grassland Species Under Combined Heat and Water Stress

Harvest-related traits are key agronomic traits for cereal crops. Understanding the influence of abiotic stress on yield and the ability to find the most predictive traits for final yield are important for understanding and characterizing crop resilience strategies. Using a high spatial and temporal phenotyping approach with multiple imaging sensors we monitored the growth dynamics of plants and assessed their physiological responses throughout development until reaching the maturity stage under control and drought conditions. Here we present a dataset comprising 145 traits over 70 time points under two conditions for six barley lines including elite, cultivated, and wild lines. We applied advanced data analysis using machine learning approaches for effective integrative visualisation and subsequent modelling. The main objectives of this study were to identify distinct traits that differentiate drought-stressed from well-watered plants and to accurately predict harvest traits. Using a classification model, we observed an accurate separation between control and drought treatments and ranked the tolerance levels of different lines. For the harvest prediction models, we evaluated all time points under different watering regimes compared with models using only early time points and determined the importance of traits. Finally, we analysed the variance components of highly predictive traits and identified which traits are mostly driven either by genetic, environmental or time components. With the applied modelling approach, we were able to predict the harvest-related traits and pinpoint the most predictive traits at specific time points. Identifying predictive traits early in the developmental stage can help breeders find stress-tolerant traits of interest.

Integrative phenotyping combined with modelling as a tool for predicting harvest-related traits

Traditional plant phenotyping for physiology, morphology, chemical composition and plant or seed biomass is labor-intensive and difficult to scale up. Like other scientific fields, it has benefited from automation, both in controlled environments and in the field. For practical reasons – particularly the need to handle the large numbers of plants required for genetic analysis - choices generally have been to measure plants fast and non-destructively. As a result, the traits being measured have shifted from ‘hard-core variables’ such as CO2  uptake rates or total plant biomass, to proxies like fluorescence or digital biomass. In this talk, I will discuss some of the implications of this shift for interpretating experimental results.
Another challenge is the gap that often exists between results obtained in controlled conditions and those observed in the field. This issue becomes even more pressing when using expensive high-throughput phenotyping equipment.  Addressing this discrepancy requires greater attention from the phenotyping community. We will also explore strategies to bridge this gap.

Premium content

Downloads

Next from SEB Conference 2025

Disentangling evolutionary and plastic responses to over-fishing of Malawi Cichlid fish in a common garden experiment

Similar lecture

METABOLOMIC CHARACTERIZATION OF AUTOCHTHONOUS LENTIL ECOTYPES