Czechia

Microplastics (MPs), defined as plastic particles smaller than 5 mm in size, are considered a global environmental and human health problem, due to their ubiquity and persistence in the aquatic ecosystems. Considering the problematic of MPs, the present study aimed to assess the toxicological effects of MPs and copper (Cu) in zebrafish brain. Polyethylene (PE) and polystyrene (PS) MPs were selected because of their extensive commercial use and high accumulation in aquatic environments.
For this purpose, zebrafish were exposed for 21 days to Cu (25μg/L), PE and PS (1mg/L) and their mixtures (PE-Cu and PS-Cu). After 21 days, fish behavior was assessed, followed by their euthanasia with an overdose of buffered tricaine methanesulfonate, and excision of the whole brain for the biomarker’s evaluation. The biochemical parameters related to oxidative stress [Reactive oxygen species, lipid peroxidation]; antioxidant activity [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), reduced (GSH) and oxidized states (GSSG) of glutathione levels]; and detoxification (glutathione-S-transferase) were assessed.
Data showed that the exploratory/locomotor, anxiety-related, and social behaviors were not significantly affected by Cu, PE, PS, and the respective mixtures. Considering the biochemical parameters, the GPx activity was significantly decreased in PE-Cu, in relation to the control group.
Considering our findings, the long-term behavioral and biochemical alterations in response to heavy metals and MPs, and its implications in fish population fitness still need to be clarified.

This work is supported by National Funds by FCT - Portuguese Foundation for Science and Technology, under the project UIDB/04033/2020.

SEB Conference 2024

The effect of polyethylene and polystyrene microplastics combined with copper in behavior and biochemical biomarkers of zebrafish (Danio rerio) brain

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SEB’s Annual Conference is celebrated for creating the perfect composition of research, new discoveries and collaborative connections. Be creative and innovative by exchanging ideas and knowledge with masters of biology from all over the world.

poster

Several snake species sport horn-like appendages over their snouts and/or eyes. Although these structures have recently attracted scientific attention, their functional significance remains unknown. One hypothesis holds that the cephalic appendages may work as heat radiators/dissipators, keeping temperature stable during sudden changes in heat load. Following this hypothesis, we expect that horns heat and cool at different rates than the rest of the head and have a stabilizing influence on brain temperature. To test this idea, we used water-filled 3D-printed head models of vipers, the snake family with the highest number and diversity in horned species. After validating our method, we compared the heating and cooling of an array of hornless and horned models in a temperature-controlled environment and monitored horn and head temperatures. Our preliminary results show that horns indeed heat and cool at different rates than the rest of the head. However, the brain region of models with and without horns heated and cooled at similar rates. Therefore, unless they are accompanied by a special blood vessel cooling and countercurrent exchange system, the horn-like structures of snakes probably cannot function to regulate head/brain temperature. We are currently investigating the possibility of such rete mirabile in snakes, using micro-CT scanning and by monitoring heating and cooling behaviour of snakes in-vivo.

Thermal properties of horns in vipers - A preliminary but innovative study using 3D-printed models

While microplastics (MPs) have become the focus of an increasing amount of research in the last decade, there is still limited knowledge on the effects these pollutants have on the physiology of aquatic organisms. The aim of this study was to determine how exposure to polyethylene (PE) microplastics (38-42 µm, 250µg/L) impacts the physiology of two representative fish species; zebrafish (Danio rerio) and killifish (Fundulus heteroclitus) under different conditions. Behavioral changes and ammonia excretion rates were compared between zebrafish exposed to MPs and controls. Killifish were examined during acclimation to changing salinity with and without the added stress of MPs exposure. Zebrafish exposed to MPs showed a significant increase in ammonia excretion rates (p=0.042) compared with controls. Behavioral changes due to MPs exposure varied, showing significant differences in surfacing (p=0.021) and hyperventilation (p=0.036) behaviors. During salinity acclimation of killifish, MPs exposure eliminated the normal increase in drinking rates commonly seen in euryhaline fishes moving into higher salinity. This type of effect would have a significant impact on the capacity of killifish to rapidly acclimate to changing salinity. 


Impact of microplastics exposure on the physiology of zebrafish and killifish

Hummingbirds have unique physiological and morphological traits to fly at high speeds and hover in place. These adaptations require a high rate of metabolism, which generates body heat as a byproduct that must be dissipated. Dissipating heat can become increasingly challenging when the thermal gradient between the bird and surrounding air becomes smaller. I aim to characterize body surface temperature patterns across the hummingbird under different temperatures and flight durations. Since the feet are relatively uninsulated, we hypothesize that the feet region is a site for efficient thermal regulation during high metabolic activity. Anna’s hummingbirds (Calypte anna) were placed under four temperatures (20℃, 25℃, 30℃, 35℃) and flew for a period of 30, 60, 90, and 120 seconds. Thermal imaging was used to record surface body temperatures prior to and immediately after each exercise bout. We find that the feet have the greatest change (10℃ rise) between the lowest and highest temperature treatments, compared to other heat-dissipating areas such as the eyes and axial region which rise by 3-5℃. Moreover, the feet showed a slight increase in surface temperature as the flight duration increased at 30℃ and 35℃, while other areas did not change. Our results demonstrate that the feet are highly flexible thermoregulatory areas during post-exercise heat dissipation. Our future work will examine the bill’s importance, another poorly insulated region of the body.


The impact of exercise and high ambient temperature on heat dissipating areas in Anna's hummingbirds (Calypte anna)

Mussels from the Mytilus edulis species complex experience a salinity gradient from the North Sea into the Baltic Proper ranging from 32 to 5 practical salinity units. As osmoconformers, mussels adjust their internal osmolarity to match that of their surroundings. This adaptive strategy poses a significant challenge to the metabolic machinery of the mussels, including their mitochondria. Thus, populations from different sites have adapted to reach their highest mitochondrial function at a salinity close to their respective habitat salinity. We hypothesised that this adaptation is accompanied by a change in accumulated metabolites. To test this hypothesis, mussels from three populations along the salinity gradient were assessed: north Baltic Sea (M. trossulus zone), south Baltic Sea (transition zone) and North Sea (M. edulis zone). Metabolites from gill tissue were measured via liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and their abundances analysed using a principal component analysis (PCA) and pathway enrichment analysis (MetaboAnalyst 6.0). The measured metabolites separated the populations from one another either by osmoregulation or by energy metabolism. Additionally, the pathway enrichment analysis identified three pathways that differed between each population. γ-aminobutyric acid (GABA) was one of the main drivers of the population specific differences, along with amino acids that are directly or indirectly connected to the citrate cycle. The citrate cycle in turn feeds the mitochondrial electron transport system (ETS). Overall, our findings suggest population specific differences in drivers of the citrate cycle as well as differences in the utilisation of sources for the mitochondrial respiration.

Metabolite profiling reveals energetic and osmoregulatory differences in populations from three zones of the Mytilus edulis species complex distribution area

The architectural properties of skeletal muscle provide an anatomical estimate of their functional capabilities. Gross dissection has long been the gold standard for exploring these variables, yet this technique has several inescapable drawbacks: being both irreversibly destructive and requiring the removal of a muscle from its original context for analysis thereby losing valuable spatial data. To counter these hurdles, several novel digital methodologies have emerged. Among these, diffusible iodine-based contrast-enhanced computed tomography (DiceCT) has been widely adopted to allow the mapping of individual fascicles in situ - enabling analysis of both their orientation and compression (i.e., tortuosity). Here, we present a cross-methodological analysis of four independent techniques for the reconstruction of muscle architectural variables: traditional gross dissection, manual segmentation of fascicles from DiceCT datastacks, and two distinct automated workflows (i.e., using Amira’s XFiber and the R package GoodFibes) using these same digital datasets. We applied these methods to a sample of eight mammals, ranging in body size from 0.1 to 10kg. First, we ran sensitivity analyses on our automated techniques to produce a single replicable workflow that could be applied across all taxa. Using this, we then calculated generally strong convergence in fascicle lengths between gross dissection, manual segmentation, and XFiber, while GoodFibes yielded consistently longer fascicles than would be expected. Both automated techniques also capture less fascicular tortuosity than manual fascicle segmentation. We conclude that automated methods represent a promising workflow to both enhance the speed of fascicular analysis and reduce user subjectivity and potential bias.

Multi-method analysis of three-dimensional techniques for the reconstruction of muscle architecture

Understanding how tiny organisms store and release energy through deformations of geometrically complex structures, such as the elastic head capsule of trap-jaw ants, is largely limited by the optical technology available to visualize small deformations across large surfaces within the mesoscale of living organisms (i.e., mm – cm). In this study, we develop an ultra-high-speed fourier light-field microscope capable of viewing specimens at high-resolution and frame rates of up to 1MHz+ (Photron E980S). In addition to validating the new instrument, we test its capabilities using Odontomachus brunneus trap-jaw ants, which can snap their mandibles shut in 0.08 ms, roughly 1000 times faster than the blink of an eye. These incredibly fast mandible strikes are possible through the slow loading and rapid unloading of elastic potential energy of the ant’s head. The head capsule deforms to store elastic potential energy during loading. During a strike, the head capsule recoils, transforming elastic potential energy stored through three dimensional deformations of the head into the rotational kinetic energy of the mandibles which move in a single plane. Using the new instrument, we successfully reconstruct the three-dimensional architecture of a portion of the head capsule during spring-loading and provide first measurements of real-time deformations in this portion of the head during unloading. These direct measurements of tiny deformations over larger fields in a structurally complex organism open a new realm for understanding energy flow and control in spring-propelled systems.

Visualization of tiny, springy deformations of an ultrafast trap-jaw ant's propulsive head

– Aposematism is a defensive strategy that combines a deterrent with visual signals. High intra-species variation in learning time to avoid the signal is seen, and this variation in behaviour influences prey defense evolution. Here, we aim to investigate if the micro-gut biome is a driver in maintaining this heterogeneity. Research with mammals establishes a relationship between gut microbiome, diet and behavior. We ask if this relationship impacts ecosystems and test if there is a feedback loop between the predator gut microbiome, cognition, foraging decisions and concentration of prey toxins consumed, and how this influences the selection pressures on chemical defenses. This provides multiple objectives: First, testing the association between the predator gut microbiome and foraging behaviour, quantifying the effect of consuming aposematic prey on the predator gut microbiome, identifying the defensive toxins that impact the predator gut microbiome, measuring the causal effect of the gut microbiome on behavior and then testing the effect of the early-life gut microbiome on foraging behaviour in adulthood. The model predators employed will be wild-caught great tits (Parus major), that will undergo altered gut microbiomes, through prey toxins and faecal microbiota transplantations. The microbial gut composition will be measured, and behavioral experiments will assess avoidance learning and dietary awareness. This investigation will deepen our understanding of the role of the predator gut microbiome in aposematism and uncover insights into the variation in predator behavior. Additionally, this investigation can propose if the gut microbiome is a factor in shaping ecology and evolution. 


The gut microbiome as a driver of foraging behaviour in predator-prey interactions.

Understanding how osmotic stress affects the performance of marine organisms is essential to produce sound predictions about the functioning of estuarine and coastal ecosystems with floods being more frequent and intense due to global changes. The green sea urchin Strongylocentrotus droebachiensis, an important top-down controller for coastal macroalgae, prefers salinities between 25 and 30; > 7 days at salinity 15 being its documented tolerance limit. Consequently, we aimed to characterize the effects of hypoosmotic stress on the physiological and behavioural responses of sea urchins during periods of flooding. We conducted a laboratory experiment along a gradient of six salinities (10, 13, 16, 20, 24 and 28 ppt), for one and two weeks. We evaluated survival, coordination performance of podia, podia adhesion, feeding rate, excretion rate, and coelomic fluid acid-base and osmotic balance following these exposure phases and following three days of recovery under control salinity conditions (28 ppt), . Urchins exposed to salinity 10 and 13 ppt experienced mortality throughout exposure, whilst survival was not compromised at salinities 16 to 28 ppt. Our results confirm an alteration of the physiological status of urchins via acid-base and osmoionic alterations and a reduction in feeding and excretion rates indicating behaviour modification. These imbalances were accompanied by a reduction in podia coordination and adhesion strength, which can lead to easier dislodgment due to waves or predators. This could ultimately lead to compromised survival and ecosystem consequences.

Characterization of the effect of hypoosmotic stress caused by coastal floodings on the physiology and behaviour of the Green Sea Urchin

Debate surrounds the role of behaviour in animal physiology, divided between traditional views that separate behaviour and physiology and a progressive perspective that supports the notion of behaviour as an inherent physiological effector. We advocate for the latter, and in this poster we provide a mechanistic and motivational perspective on behaviour and draw parallels with recognised physiological processes. Indeed, behaviour is ultimately generated by spatial and temporal patterns of musculoskeletal activity and it is tightly regulated by neural pathways, like other physiological processes. For example, many bird species will employ vasoconstriction and shivering – two well-established physiological processes in thermoregulation – and social huddling behaviour to avoid hypothermia. Yet, analogous neuroendocrine patterns can be observed in all three of these physiological actions. Furthermore, these responses are elicited by an animal’s motivational state in response to challenges in its environment. This motivational state can trigger a variety of physiological responses – behavioural or otherwise – that, while operating concurrently, all aim to satisfy an underlying ‘need’. For example, when an animal’s water balance approaches critical levels, it may employ a suite of physiological actions/strategies to conserve (e.g., water reabsorption in the gut, behavioural thermoregulation) and acquire water (e.g., water vapour absorption, drinking/feeding), driven by a motivational state to avoid osmotic stress. Our goal with this poster is to set the stage for a debate and persuade physiologists of the merits of including behaviour within the domain of animal physiology. Recognising behaviour as a physiological process is crucial for advancing a unified understanding of physiology.

Scaling organismal physiology: a case for the integration of behaviour as a physiological process

An important trait of Pacific bluefin tuna (PBT) is its ability to maintain body temperature through high metabolic heat production and its retention ability. PBT rapidly develop the thermo-conservation ability between 20 and 40 cm fork length (FL), which has been attributed to improved insulation. Meanwhile, how heat production capacity develops during ontogeny is not well understood. The aim of the present study was to explore the ontogenetic change in metabolic heat and its contribution to endothermic ability through swim-tunnel respirometry and a heat-budget model. Swim-tunnel respirometry was conducted on PBT, ranging from 19.4 to 27.5 cm FL (94–330 g, n=16) in Kochi Prefecture, Japan, from August to September 2022 and 2023. The metabolic rate of each PBT was calculated as the standard metabolic rate (BSMR) at a swim speed of 0 cm/s and the minimum metabolic rate (BUmin) at a minimum swim speed of 44.3 cm/s. The scaling exponents of BSMR and BUmin were estimated at 1.03 and 0.93, respectively. Both scaling exponents of metabolic rates were higher than those estimated for the tribe Thunnini above 300 g. Furthermore, a scaling exponent close to 1 implies that heat production rate (Tm in °C/min) in heat-budget model does not decrease in this body size range. To explore the ontogenetic change of heat-production rate of PBT in nature, heat-budget model was applied to bio-logging data collected from nine juveniles (size of analysis: 23–60 cm FL; 200–5272 g) with electronic archival tags inserted into their body cavity. The heat-production rate of each PBT did not decrease until approximately 800 g and declined thereafter, supporting the results of swim-tunnel respirometry. Furthermore, we evaluated the effects of heat production rate on the thermo-conservation ability, the difference between body temperature and water temperature, and found that maintaining a high heat production rate mainly contributed to the initial stage of temperature difference generation. Our results provided new insights into the endothermal development of tuna.

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