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Sepsis is a life-threatening state of disseminated infection, and treatment requires knowledge of the organism responsible. The gold standard for sepsis diagnosis is blood culture, which requires days of growth. Next-generation sequencing has been proposed as an alternative; however, existing methods may lack sensitivity. We validated the RNA quasi-G-prime classification against cell culture and showed that RNA quasi-G-primes reliably differentiate septic states from healthy states in humans.
